13792

Real-time multi-view deconvolution

Benjamin Schmid, Jan Huisken
Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
arXiv:1503.07998 [q-bio.QM], (27 Mar 2015)

@article{schmid2015realtime,

   title={Real-time multi-view deconvolution},

   author={Schmid, Benjamin and Huisken, Jan},

   year={2015},

   month={mar},

   archivePrefix={"arXiv"},

   primaryClass={q-bio.QM}

}

In light-sheet microscopy, overall image content and resolution are improved by acquiring and fusing multiple views of the sample from different directions. State-of-the-art multi-view (MV) deconvolution employs the point spread functions (PSF) of the different views to simultaneously fuse and deconvolve the images in 3D, but processing takes a multiple of the acquisition time and constitutes the bottleneck in the imaging pipeline. Here we show that MV deconvolution in 3D can finally be achieved in real-time by reslicing the acquired data and processing cross-sectional planes individually on the massively parallel architecture of a graphics processing unit (GPU).
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